178 BD FACSCalibur Instructions for Use
Changing the Sample O-ring
The sample tube O-ring is located within the outer sleeve retainer. It forms a seal
that allows the droplet containment vacuum to function properly. Replace the
O-ring when droplets form at the end of the SIT while the vacuum is operating.
1 Remove the outer sleeve from the SIT.
See Figure 8-2 on page 176.
2 Invert the retainer and tap out the O-ring.
3 Drop a new O-ring into the retainer.
Make sure the O-ring is seated properly in the bottom of the retainer.
4 Reinstall the retainer and the outer sleeve.
Tighten the retainer enough to hold it in place, and slide the outer sleeve
over the SIT, into the retainer opening. Push the sleeve up as far as it goes as
you continue tightening the retainer.
5 Install a sample tube on the SIP.
If the sleeve hits the bottom of the tube, loosen the retainer slightly, and
push the sleeve up. Retighten the retainer.
Any instrument surface that comes in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when cleaning
the instrument or replacing parts. Wear suitable protective clothing,
eyewear, and gloves.
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