200 BD FACSCalibur Instructions for Use
BD Multiset Troubleshooting
The following are the most common error codes that appear on Lab and
Physician Reports when acquisition criteria are not met.
NOTICE When an error code is generated, BD Multiset software creates a Bad
Files folder within the folders for Lab and Physician Reports and data files. The
Bad Files folder contains aliases (shortcuts) to files with QC flags.
Error Codes
Code Error Message Solution(s)
1 Could not acquire
the user-requested
[x number of
population] events
1 Decrease the Min Events to Acquire in
Reagent Tools (Acquisition Target Info).
2 Adjust acquisition stopping criteria in Events
Preferences.
3 Adjust acquisition target population gate or
attractor at Manual Gate view, if necessary.
4 Increase threshold if there is excessive debris.
2 Could not acquire
the BDIS-preferred
[500 bead] events.
1 Adjust acquisition stopping criteria for user-
requested number of events in Events
Preferences.
2 Visually inspect. Adjust gate to fully capture
bead population within attractor at Manual
Gate view, if necessary.
3 Increase threshold if there is excessive debris.
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