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4
Instrument QC
To ensure the flow cytometer provides consistent results, run BD FACSComp
software with BD Calibrite beads at the start of each workday. The software
adjusts detector voltages to place beads at target channel values and performs a
Sensitivity Test to compare signal separation for each parameter to an expected
minimum value. Log or export the summary data to track instrument
performance over time. For a sample log, see page 83.
NOTICE Because leucocytes have different optical properties than BD Calibrite
beads, it is important to optimize settings for cell samples. You can optimize
settings in BD FACSComp, BD CellQuest Pro, or BD Multiset software.
This chapter describes how to perform instrument quality control (QC) and
optimize settings using BD FACSComp software.
• Setting Up for QC on page 62
• Running BD FACSComp Software on page 66
• Optimizing Settings on page 79
• Logging QC Results on page 83
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