Chapter 5: Running Samples Manually 133
Reviewing Lab Reports
Although BD Multiset is a very sophisticated software, a technologist must
review all data plots before signing the results. The operator should verify that
adequate lymphocyte events were collected, and that attractors are correctly
placed. If an insufficient number of lymphocytes were collected (ie, less than the
BD or user-defined criteria), the sample must be reacquired with a new FL3
threshold to increase the number of collected cells. The new threshold is
acceptable as long as it does not cut through the lymphocyte population.
If you are running Acquisition With Analysis, the Lab Report view appears after
each tube is run. The view pauses for the amount of time specified in Set Up; you
can inspect plots during the pause.
expert lymph gate
attractor gate
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