134 BD FACSCalibur Instructions for Use
After previewing the report, use manual gating to adjust the expert lymph gate or
attractors, if needed. For results troubleshooting, see page 203.
Manual Gating
NOTICE Changes made to the gate, attractors, or display are applied to the
current tube only.
1 Click Manual Gate.
2 To re-draw the expert lymph gate, double-click the CD45 vs SSC plot.
3 Create a polygon region around the lymphocytes.
• Move the cursor near the lymphocytes and click to start the region.
• Click around the population of interest until the region is complete.
• Close the region by double-clicking the last vertex or clicking the first.
4 Click the close box.
5 Select any attractor gate to adjust it.
To Next Page
Loading...
