Chapter 9: Troubleshooting 197
Time Delay Setup
a
a. Applies only for 4-color setup for instruments with the FL4 option.
Observation Possible Causes Recommended Solutions
Time-delay
calibration fails
Air bubble in flow cell Prime the fluidics (page 58).
Cracked sample tube or
pressure leak from tube
Check the tube and seal; replace
as needed (page 176).
Low event rate for APC
beads
• Mix tube.
• Set the flow rate to HI.
• Add APC beads to the
unlabeled + APC bead tube.
Loose sheath cap Tighten the sheath cap.
Empty sheath reservoir Fill the sheath reservoir.
Incorrect APC bead lot ID Enter the correct APC bead lot
ID.
FL4 PMT set incorrectly Adjust settings manually for
PMTs without checkmarks
(page 71, step 4).
FSC signal < channel 160 Contact BD Biosciences.
FL4 PMT mean channel
changed by >5 channels
after time-delay calibration
Rerun the software.
Air in sheath filter Vent air from the sheath filter.
See page 57, step 14.
Decreased sheath pressure
from loose cap or leaking
reservoir
• Ensure the sheath cap is tight
and all connectors are secure.
• Check for leaks or cracks in
the sheath reservoir. Replace
it, if needed.
• Check the Status sample
voltage, in RUN with test
tube off (page 112).
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