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BD FACSCalibur - Page 57

BD FACSCalibur
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Chapter 3: Starting Up 57
9 Replace and tighten the cap assembly.
10 Install the reservoir, and replace the metal bracket.
Slide the metal bracket toward you. Make sure the square tab on the right
side of the bracket is covering the ball valve.
11 Reconnect the sheath and air supply tubing to their color-coded fittings.
12 Reconnect the level sensor line.
13 Pull the vent valve toward you, and verify the tank is pressurized.
The sheath reservoir should not move under the metal bracket.
14 Check the sheath filter for air bubbles.
If you see bubbles, gently tap the filter body to dislodge bubbles and force
them to the top. Push the roller in the pinchcock forward to force the
bubbles to waste. Close the pinchcock. Repeat, if necessary.
15 Check the sheath line and the sheath filter line for bubbles.
If bubbles are in either line, disconnect the tubing and press the tip of the
connector valve against the side of a waste beaker. Pressurized sheath fluid
will force bubbles and sheath fluid out of the tubing.
Do not overfill the sheath reservoir. Pressure on an overfull tank can force
fluid into the air supply tubing, preventing proper pressurization.
Furthermore, because of the additional bleach in the waste reservoir,
filling the sheath to maximum capacity can overflow the waste.
Make sure the cap is securely tightened and the tubing is not pinched or
twisted. If the cap is loose or the tubing is kinked, sample flow could be
lessened which will affect your results.

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