Chapter 9: Troubleshooting 191
Acquisition Troubleshooting
Observation Possible Causes Recommended Solutions
No events displayed
and Status = READY
Insufficient gain for
threshold parameter
Increase the Amp Gain for the threshold
parameter (page 90).
Threshold too high Lower the threshold.
Threshold set to
incorrect parameter
Set the threshold to the appropriate
parameter for the application (usually
FSC). See page 92.
No sample in tube Add sample or install new sample tube.
Improperly mixed
sample
Mix sample to suspend cells.
Clogged SIT • Remove the sample tube to allow
backflushing.
• If the clog persists, clean the SIT
(page 165).
Gated plot with no data
in gate
Delete or move the gate.
Plot type = Analysis In the Inspector, change the Plot Type to
Acquisition or Acquisition-to-Analysis.
Worn Bal seal Replace the Bal seal (page 176).
Communication failure
between computer and
instrument
1 Turn off the computer and instrument.
2 Turn on the instrument, then the
computer.
GPIO error: cannot read
instrument status
1 Turn off the computer and instrument.
2 Reseat the GPIO cable (Figure 1-10 on
page 22).
3 Turn on the instrument, then the
computer.
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