Chapter 4: Instrument QC 69
11 When the last assay is complete, optimize settings for cell samples if needed
(page 79).
12 At the Set Up or Summary view, click Quit.
If you selected the Automatic Clean preference, cleaning begins.
13 When cleaning is finished, remove the Loader rack, install a tube
containing no more than 1 mL of DI water on the SIP, and press STNDBY.
Acquiring Manually
1 Gently mix the unlabeled (or unlabeled and APC) bead tube, and install it
on the cytometer.
2 On the cytometer, press RUN and HI.
3 At the Set Up view, click Run; at the PMT view, click Start.
BD FACSComp software acquires 5,000 events and sets a singlet gate for
FSC intensity. After the gate has been set, the software starts automatic
PMT voltage adjustments. For instruments with the FL4 option, the
software looks for APC beads.
• If no APC beads are found, the second laser is turned off and PMTs are
adjusted for 2- or 3-color setup.
• If APC beads are found and an APC bead lot ID was entered at the Set
Up view, the second laser remains on, and the FL4 PMT is adjusted.
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