BD FACSCalibur - Modifying the Cytometer for Non-Loader Use

186 BD FACSCalibur Instructions for Use
For other troubleshooting suggestions, see page 209.
Modifying the Cytometer for Non-Loader Use
When a Loader is installed, the Bal seal is removed and a Loader seal and tube
guide are installed. This configuration creates the proper seal and pressurization
for Loader use. To acquire samples without the Loader, you must remove the
tube guide and replace the Bal seal.
1 Turn off the instrument main power.
2 Remove the tube guide retainer and attached tube guide.
Turn the tube guide and tube guide retainer counterclockwise, as shown.
Any instrument surface that comes in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when cleaning
the instrument or replacing parts. Wear suitable protective clothing,
eyewear, and gloves.
tube guide retainer
tube guide