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FACSCalibur
BD FACSCalibur User Manual
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149
6
Running Samples with the Loader
Before you
begin, start up
the cytometer (page
5
5), perform in
strument QC
(page
61) an
d optimi
ze settings for sam
ples to b
e run (page
7
9).
•
Preparing an Acqu
isition Documen
t on page
15
0
•
Setting Up BD W
orklist Manager Software on page
151
•
Entering Sample In
formation on page
156
•
Running Samples on page
160
148
150
Table of Contents
Default Chapter
5
Table of Contents
5
About These Instructions
11
Conventions
11
Chapter 1: Introduction
13
System Components and Theory of Operation
14
Cytometer Components
15
Loader (Optional)
20
Power Controls
22
System Requirements
23
Chapter 2: BD Facstation Software
25
BDPAC Software
27
BD Cellquest Pro Software
28
BD Cellquest Pro Files
30
BD Facscomp Software
31
BD Facscomp Preferences
33
BD Facscomp Files
34
BD Multiset Software
39
BD Multiset Preferences
41
BD Multiset Tools
43
BD Multiset Files
45
BD HLA-B27 Software
47
BD HLA-B27 Preferences
49
BD HLA-B27 Files
50
BD FACS Loader Software
51
Worklist Manager Preferences
53
Worklist Manager Files
53
Chapter 3: Starting up
55
Filling the Sheath Reservoir
56
Priming the Fluidics
58
Emptying the Waste Reservoir
58
Chapter 4: Instrument QC
61
Setting up for QC
62
Editing Target Values
65
Running BD Facscomp Software
66
Acquiring with the Loader
66
Acquiring Manually
69
Viewing Results
77
Optimizing Settings
79
Downloading Instrument Settings
82
Logging QC Results
83
Chapter 5: Running Samples Manually
85
Running Samples with BD Cellquest Pro Software
86
Setting up an Experiment Document
86
Optimizing Settings
90
Acquiring Data
99
Saving Data
110
Analyzing Data
113
Running Samples with BD Multiset Software
121
Setting up
121
Acquiring Data
130
Reviewing Lab Reports
133
Analyzing Data
137
Running Samples with BD HLA-B27 Software
139
Setting up
139
Acquiring Data
144
Analyzing Data
146
Chapter 6: Running Samples with the Loader
149
Preparing an Acquisition Document
150
Setting up BD Worklist Manager Software
151
Setting up Assay Settings
153
Running BD Facscomp Software
155
Saving the Worklist
155
Entering Sample Information
156
Assigning Racks
158
Running Samples
160
Pausing Acquisition
163
Rerunning a Sample
164
Chapter 7: Shutting down
165
Daily Shutdown with the Loader
166
Chapter 8: Maintenance
169
Scheduled Maintenance
170
Decontaminating the Fluidics
171
Changing the Sheath Filter
173
Unscheduled Maintenance
175
Changing the Bal Seal
176
Changing the Sample O-Ring
178
Loader Maintenance
179
Checking Tube Lifter Calibration
180
Verifying Connection Ports
181
Replacing the Loader Seal
182
Cleaning the Tube Lifter
184
Modifying the Cytometer for Non-Loader Use
186
Chapter 9: Troubleshooting
189
Instrument Troubleshooting
190
Acquisition Troubleshooting
191
BD Facscomp Troubleshooting
195
PMT Adjustment
195
Time Delay Setup
197
Fluorescence Compensation
198
Sensitivity Test
199
HLA-B27 Calibration
199
BD Multiset Troubleshooting
200
Error Codes
200
General BD Multiset Software
201
BD Multiset Results
203
Loader Troubleshooting
208
Worklist Manager Troubleshooting
210
Appendix A: Supplies and Replacement Parts
211
Instrument Supplies
212
Accessory Kit
212
Other Replacement Parts
213
Consumables
214
Instrument Setup
214
Reagents
215
Labware
215
Appendix B: Technical Specifications
217
Cytometer Specifications
218
Environment
219
Performance
219
Optics
220
Fluidics
221
Signal Processing
222
Loader Specifications
222
Connections
223
Appendix C: Performance Data
225
Accuracy
226
Precision
227
Linearity
229
Index
231
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BD FACSCalibur Specifications
General
Brand
BD
Model
FACSCalibur
Category
Measuring Instruments
Language
English
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