Chapter 1: Introduction 15
Cytometer Components
Figure 1-2 BD FACSCalibur sensor unit
Fluidics Components and Theory of Operation
You install tubes onto the sample injection port (SIP). Samples are carried to the
flow cell through a stainless steel sample injection tube (SIT) surrounded by a
droplet containment sleeve. The sleeve works in conjunction with a vacuum
pump to eliminate droplets as fluid backflows from the SIT. The vacuum pump is
activated by the tube support arm—the vacuum is on when the arm is to the side
and off when the arm is centered.
Figure 1-3 Sample injection port (SIP)
fluidics control panel
fluidics
sample injection port
power switch
drawer
sample injection tube
droplet containment sleeve
tube stop
tube support arm
(SIT)
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