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FACSCelesta Flow Cytometer
BD FACSCelesta Flow Cytometer User Manual
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23-17147-0
2
9/2020
For Research Use Only
Becton, Dickinson and Company
BD Biosciences
2350 Qume Drive
San Jose, CA 95131 USA
bdbiosciences
.
com
ResearchApplications@bd.com
BD Biosciences
European Cust
omer Support
Tel +32.53.720.600
help.biosc
iences@bd.
com
BD FACSCelesta™ Flow
Cytometer
User’s Guide
2
Table of Contents
default chapter
5
Table of Contents
5
Chapter 1: About this Guide
9
What this Guide Covers
10
Conventions
11
About the BD Facscelesta Documentation
11
Instrument Technical Support
13
Chapter 2: Introduction
15
System Overview
16
Cytometer Overview
17
Control Panel
19
Fluidics System
20
Sheath and Waste Containers
27
Optics
28
Workstation
30
Chapter 3: Cytometer Setup
31
Starting the Cytometer and Computer
32
Preparing the Sheath Container
33
Removing Air Bubbles
35
Preparing the Waste Container
38
Priming the Fluidics
40
About the Optical Filters and Mirrors
41
Custom Configurations and Baselines
43
Chapter 4: Maintenance
45
Maintenance Overview
46
Cleaning the Fluidics
47
Shutting down the Cytometer
49
Flushing the System
50
Replacing the Waste Container Cap
52
Changing the Sheath Filter
54
Changing the Bal Seal
56
Changing the Sample Tube O-Ring
58
Cleaning or Replacing the Sheath Gasket
60
Chapter 5: Optimizing Cytometer Settings
61
Cytometer Settings Workflow
62
Verifying the Configuration and User Preferences
64
Running a Performance Check
67
Setting up an Experiment
72
Creating Application Settings
77
Recording Compensation Controls
80
Calculating Compensation
83
Chapter 6: Recording and Analyzing Data
85
Data Recording and Analysis Workflow
86
Preparing the Workspace
87
Recording Data
88
Analyzing Data
91
Reusing an Analysis
97
Chapter 7: Technical Overview
99
About Fluidics
100
About Optics
101
About Electronics
112
Chapter 8: Troubleshooting
117
Cytometer Troubleshooting
118
Electronics Troubleshooting
126
Chapter 9: Detector Array Configurations
127
Fluorescence Spectra
128
About Configuration Maps
131
About the Configuration
132
Base Configuration Polygon Maps
134
Chapter 10: Supplies and Consumables
143
Ordering Information
144
Beads
144
Reagents
145
Equipment
146
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BD FACSCelesta Flow Cytometer Specifications
General
Brand
BD
Model
FACSCelesta Flow Cytometer
Category
Measuring Instruments
Language
English
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