Chapter 7: Technical overview 101
Changing the sample flow rate changes the sample pressure
thereby changing the core diameter. The flow rate should be set
according to the type of application you are running.
• A higher flow rate is generally used for qualitative
measurements such as immunophenotyping. The data is less
resolved, but is acquired more quickly.
• A lower flow rate is generally used in applications where
greater resolution and quantitative measurements are critical,
such as DNA analysis.
Proper operation of fluidic components is critical for particles to
intercept the laser beam properly. Always ensure that the fluidics
system is free of air bubbles and debris, and is properly
pressurized.
About optics
Introduction This topic describes the optics system and provides information
about:
• Light scatter (page 102)
• Fluorescence (page 102)
• Optical filter theory (page 104)
• Compensation theory (page 109)
Optics system The optics system consists of lasers, optical filters, and detectors.
Lasers illuminate the cells or particles in the sample and optical
filters direct the resulting light scatter and fluorescence signals to
the appropriate detectors.
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