BD FACSCelesta Flow Cytometer - Data recording and analysis workflow

BD FACSCelesta Flow Cytometer User’s Guide86
Data recording and analysis workflow
Introduction This topic outlines the basic acquisition and analysis tasks using
BD
FACSDiva software.
About the
examples
The examples in this chapter are from two 4-color bead samples
with the following fluorochromes:
•FITC
•PE
•PerCP-Cy5.5
•APC
If you use a different sample type or if you have skipped the
optimization steps in
Optimizing cytometer settings (page 61),
your software window content, names of folders and experiments,
and your data plots and statistics might differ from those shown
here. You might also need to modify some of the instructions in the
procedure.
For additional details on completing some of the following steps,
see the BD
FACSDiva Software Reference Manual.
This procedure builds on the results obtained in Optimizing
cytometer settings (page 61).
Workflow for
recording and
analyzing data
Recording and analyzing data consists of the following steps.
Step Description
1 Preparing the workspace (page 87)
2 Recording data (page 88)
3 Analyzing data (page 91)
4 Reusing an analysis (page 97)