Chapter 4: Maintenance 47
When to perform
maintenance
procedures
Perform maintenance procedures in the following frequencies.
Cleaning the fluidics
Introduction This topic describes how to perform the daily fluidics cleaning.
Overview Cleaning the fluidics daily prevents the sample injection tube from
becoming clogged and removes dyes that can remain in the tubing.
In addition to daily cleaning, follow this procedure immediately
after running viscous samples or nucleic acid dyes such as Hoechst,
DAPI, propidium iodide (PI), acridine orange (AO), or thiazole
orange (TO).
Procedure To clean the fluidics:
1. Press RUN and HIGH on the cytometer fluid control panel.
2. Install a tube containing 3 mL of a 10% bleach solution on the
SIP with the support arm to the side (vacuum on) and let it run
for 1
minute.
Frequency Maintenance procedure
Daily
Cleaning the fluidics (page 47)
Shutting down the cytometer (page 49)
Scheduled (every
two weeks)
Flushing the system (page 50)
Periodic (frequency
depends on how
often you run the
cytometer)
Changing the sheath filter (page 54)
Changing the Bal seal (page 56)
Changing the sample tube O-ring (page 58)
Cleaning or replacing the sheath gasket
(page 60)
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