BD FACSCelesta Flow Cytometer - Recording data

BD FACSCelesta Flow Cytometer User’s Guide88
2. In the Application Settings catalog, select the application
settings file you saved previously and click Apply.
If the parameters are not the same, a mismatch dialog opens.
•Click Overwrite to update all settings.
•Click Apply to change only the common parameters.
For more information, see the BD FACSDiva Software
Reference Manual.
The cytometer settings are renamed application settings and the
cytometer settings icon in the Browser changes.
More information • Creating application settings (page 77)
• Recording data (page 88)
Recording data
Introduction This topic provides an example of how to preview and record data
for multiple samples.
Before you begin Prepare the sample tubes.
Note: If you need to run samples at an event rate greater than
10,000 events/second, consider changing your Window extension.
See the BD
FACSDiva Software Reference Manual for more
information.
Recording data To record data:
1. Press RUN and HIGH on the cytometer fluid control panel.
2. Install the first sample tube onto the SIP.
3. Set the current tube pointer to Beads_001.