Chapter 3: Cytometer setup 35
5. Remove the sheath container lid.
Unscrew the clamp knob and push down to loosen, if
necessary. Tilt the cap to the side to remove it from the tank.
6. Add up to 10 L of sheath fluid, such as BD FACSFlow
solution, to the sheath container.
Note: 10 L will reach the interior line on the sheath tank. Do
not fill the sheath tank further.
7. Replace the sheath container lid.
8. Make sure the gasket on the inside lip of the sheath lid is
seated correctly and has not slipped out of position.
Note: If the gasket is not seated correctly, the tank will not
pressurize properly.
9. Close the sheath lid and tighten the clamp knob to finger-tight.
10. Reconnect the green air line, alarm line and the blue sheath
line.
Note: Ensure that the blue sheath fluid line is not kinked.
More information • Removing air bubbles (page 35)
• Changing the sheath filter (page 54)
• Cleaning or replacing the sheath gasket (page 60)
Removing air bubbles
Introduction This topic describes how to remove trapped air bubbles in the
sheath filter and the sheath line. Air bubbles can occasionally
dislodge and pass through the flow cell, resulting in inaccurate
data.
Note: Perform this activity every time the sheath tank is refilled.
To Next Page
Loading...
