BD FACSCelesta Flow Cytometer - Optics

BD FACSCelesta Flow Cytometer User’s Guide28
Optics
Introduction This topic describes the optical components for the
BD
FACSCelesta flow cytometer including:
• Detector arrays
• Laser options
• Optical filters
• Signal detectors
Detector arrays The BD FACSCelesta detector arrays consist of polygons. Each
polygon can be outfitted with two to six fluorescent detectors and
can detect up to six fluorescent signals.
Laser options The BD FACSCelesta flow cytometer can be configured with up to
three lasers as listed in the following table.
Optical filters Optical filters attenuate light or help direct it to the appropriate
detectors. The name and spectral characteristics of each filter
appear on its holder.
There are two types of optical filters in the BD FACSCelesta:
• Longpass dichroic filters (LPs). Transmit wavelengths at or
longer than the specified value and reflect all light below the
specified wavelength.
Laser Wavelength (nm) Power (mW)
Violet (standard) 405 50
Blue (standard) 488 20
UV (optional) 355 15
Yellow Green
(optional)
561 50
Red (optional) 640 40