BD FACSAria User Manual (English)

Other manuals for BD FACSAria

Quick Guide5 pages

Startup, Qc & Shutdown Procedure9 pages

Questions and Answers:

C
Catherine HillAug 20, 2025
What to do if there is a high electronic abort rate on BD FACSAria Measuring Instruments?
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  Amanda WilsonAug 21, 2025
  If you observe a high electronic abort rate (greater than 10% of the system event rate) on your BD Measuring Instruments, it could be due to several factors: * The window extension might be too high. Try decreasing the window extension. * The threshold channel might be set too low. Increase the threshold channel. * The event rate could be too high. Decrease the flow rate in the acquisition dashboard. * The sample may be aggregated. Filter the sample. * The sample might be too concentrated. Dilute the sample. * The window extension might be set incorrectly. Adjust the window extension, if needed.
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Nancy KaiserAug 22, 2025
How to fix communication failure between workstation and cytometer in BD FACSAria Measuring Instruments?
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  Jessica FieldsAug 22, 2025
  If you're experiencing a communication failure between the workstation and the cytometer of your BD Measuring Instruments, a simple solution is to quit the software and then restart it.
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David RiceAug 25, 2025
What to do if there is no stream when Stream control is clicked on BD FACSAria?
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  jamesgarnerAug 25, 2025
  If there's no stream when you click the Stream control on your BD Measuring Instruments, it could be due to several reasons: * There might be air in the bubble filter. Purge the bubble filter. * The sheath container might be low. Refill the sheath tank. Note that the stream shuts off automatically if the empty tank warning message is not dismissed after 15 minutes. * There might be air in the sheath line. Prime the sheath tank. * There might be air in the sheath filter. Purge the sheath filter. * The filter may be dry. Install bypass tubing in place of the filter for the affected fluid, then run Prime After Tank Refill. Re-install the filter and open the bleeder valve to purge the filter.
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Pedro EatonAug 26, 2025
Why is there an erratic event rate on BD FACSAria?
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  aaron57Aug 26, 2025
  If you observe an erratic event rate on your BD Measuring Instruments, consider these potential causes and solutions: * Sample aggregates might be present. Filter the sample to remove aggregates. * The sample could be contaminated. Re-stain the sample, ensuring the tube is clean.
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Christine SavageAug 27, 2025
Why is there a fluid leak under the fluidics cart of BD Measuring Instruments?
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  Jake CooleyAug 27, 2025
  If you see fluid leaking under the fluidics cart or below the side door of your BD Measuring Instruments, it may be due to condensation from the pressure relief valve. This is normal when water condenses from room air, especially in humid environments. To prevent slipping, regularly check and wipe up any accumulated water.
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Mr. Jacob IngramAug 31, 2025
Why is there fanning around center stream or side streams in BD FACSAria?
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  Anthony ClarkAug 31, 2025
  If you observe fanning around the center stream or side streams on your BD Measuring Instruments, it might be because the nozzle is inserted improperly. Turn off the stream, remove the nozzle and ensure that the O-ring is in place. Re-insert the nozzle at a slight downward angle to prevent loss or movement of the O-ring, ensuring it's completely seated against the back wall of the cuvette flow cell.
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Margaret AllenSep 2, 2025
Why is there no fluorescent signal on BD FACSAria Measuring Instruments?
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  Mary CarpenterSep 2, 2025
  If you observe no fluorescent signal on your BD Measuring Instruments, it could be due to: * The current cytometer configuration differing from the optical setup. Verify that the cytometer optics match the current cytometer configuration. * The wrong filter being installed or the filter not being completely seated. Make sure the appropriate filter is installed for each fluorochrome and that the filters are pushed all the way in. * The laser delay being set incorrectly. Adjust the laser delay settings.
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Laurie DaltonSep 4, 2025
What to do for unexpected sort results in BD FACSAria Measuring Instruments?
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  Matthew SimsSep 4, 2025
  If you are getting unexpected sort results from your BD Measuring Instruments, create a new subset under the parent population consisting of NOT (child). Sort the child population into one tube and the NOT (child) population into another tube.
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Anne WhiteSep 5, 2025
What to do if fluidics cart air flow is >100 psi on BD Measuring Instruments?
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  Stephen LopezSep 6, 2025
  If the fluidics cart air flow is greater than 100 psi on your BD Measuring Instruments, contact your BD Biosciences service engineer because the regulator is not adjusted properly.
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andre70Sep 7, 2025
Why is stream control disabled on BD Measuring Instruments?
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  John ShawSep 7, 2025
  If the stream control is disabled or there's no stream when the Stream control is clicked on your BD Measuring Instruments, it could be due to: * The plenum not being full. Wait for the plenum to fill. * An air lock in the bubble or fluidics. Prime the system with the corresponding fluid. If the control is still disabled, remove the filter, install bypass tubing, and repeat the priming procedure until you see fluid in the line. When fluid is running through the line, remove the bypass tubing, install the filter, and repeat the priming procedure one last time.

BD FACSAria Specifications

General

Sorting Speed	Up to 70, 000 events per second
Laser Options	488 nm, 633 nm, 405 nm
Fluorescence Detectors	PMT detectors
Sample Collection	Tubes or plates
Nozzle Sizes	70 μm, 85 μm, 100 μm, 130 μm
Cell Size Range	1-50 μm
Sorting Modes	Single cell, bulk sort, index sorting
Data Acquisition	BD FACSDiva software