Chapter 1: BD FACSAria Cytometer Components 27
See the following sections for more information about the flow cytometer.
• Fluidics Components on this page
• Optics System on page 36
• Cytometer Electronics on page 42
Fluidics Components
When the fluidics system is activated, sheath fluid is pumped from the fluidics
cart into a plenum reservoir where the fluidics system is pressurized. From the
reservoir, fluid is pumped into the cuvette flow cell where hydrodynamic focusing
forces particles from the sample injection chamber through the cuvette in a
single-file stream.
Within the cuvette flow cell, laser light is focused on the sample core stream.
Fluorescent molecules excited by the different laser wavelengths are detected by
the optics and analyzed by the electronics. Particles are then either transported to
waste reservoirs via the waste aspirator, or sorted into a receptacle within the sort
collection chamber.
The following fluidics components are described in this section. For more
information about fluidics, see Fluid Movement on page 46.
• Plenum Reservoirs on page 28
• Sample Injection Chamber on page 29
• Cuvette Flow Cell on page 30
• Nozzle on page 31
• Sort Block on page 32
• Sort Collection Chamber on page 35
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