28 BD FACSAria User’s Guide
Plenum Reservoirs
Sheath fluid is pumped from the fluidics cart into a plenum reservoir inside the
side door of the BD FACSAria flow cytometer (Figure 1-7). From there, the fluid
travels to a second reservoir where it is held and pressurized until it flows into the
cuvette flow cell. This system eliminates pressure fluctuations by maintaining
fluid levels and filtering out pump pulsation. As a result, the fluidics pressure
does not vary with the level of fluid in the sheath containers.
Figure 1-7 Plenum reservoirs
Note that the plenum reservoirs can be unscrewed and removed for cleaning, if
needed. See Cleaning the Plenum Reservoirs on page 190.