Chapter 6: Shutdown and Maintenance 209
Cleaning the Nozzle
Use one of the following methods to clean the nozzle when the stream appears
blocked or distorted. To verify that the nozzle is clogged, examine the tip under a
microscope. Figure 6-9 shows an example of an unclogged nozzle tip.
Figure 6-9 Magnified view of a nozzle tip
1 Turn off the stream and open the flow cell access door.
2 Remove the nozzle from the cuvette flow cell.
Turn the nozzle-locking lever 90° to the left. Remove the nozzle by pulling
it straight out.
3 Remove and set aside the O-ring.
4 Sonicate the nozzle for approximately 1 minute.
Sonicate the nozzle in a test tube containing DI water or a mild detergent.
Repeat the sonication as needed until the nozzle is clean.
5 Thoroughly dry the nozzle.
All biological specimens and materials coming into contact with them can
transmit potentially fatal disease. Handle nozzles as if capable of
transmitting infection. Wear suitable protective clothing and gloves.
O-ring
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