Chapter 4: Running Samples 133
d Ensure that the red and violet laser delays are set appropriately by
setting the window extension to 0 and verifying that the area signal is
not lost. Adjust the laser delay, if necessary.
e Reset the window extension to the appropriate value before verifying
the remaining area scaling factors.
f Verify the red and violet laser area scaling factors.
6 Set the Flow Rate to 1.0.
7 Record 10,000 events.
Do not adjust any voltages. You may need to adjust the singlet gate to
capture the population in the FSC-A vs SSC-A dot plot.
Tracking QC Results
Monitoring cytometer QC results can provide you with information regarding
your instrument’s performance. Changes in mean fluorescence intensity or CV
can alert you to possible instrument issues or service procedures that may need to
area scaling values
tube tab
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