Chapter 4: Running Samples 119
Setting Up Cytometer Configuration
The cytometer configuration includes these settings:
• Fluorochromes
• Filters and mirrors
• Sheath pressure (must match sheath pressure in sort setup)
• Sort setup (sheath pressure in sort setup must match sheath pressure in
configuration)
• Nozzle size
• Window extension
The steps in this section show you how to set up cytometer configuration in
preparation for performing QC.
1 Select Cytometer > View Configurations, which opens the Cytometer
Configuration window in the CS&T module. See Figure 3-5 on page 88.
NOTE When you select Cytometer > View Configurations, the cytometer
disconnects from the BD FACSDiva interface and connects to the CS&T module.
While the CS&T module is running, BD FACSDiva is in a holding mode and does
not accept any user input. When CS&T is closed, BD FACSDiva software
becomes active again.
2 Select a cytometer configuration that contains appropriate settings for the
samples you are running.
3 Once the appropriate configuration is selected, click OK in the Cytometer
Configuration window to close it.
For accurate data results, the cytometer optics must match the current
cytometer configuration. Also, the sheath pressure and sort setup must
match the settings in BD FACSDiva software.
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