Chapter 6: Shutdown and Maintenance 203
9 Verify the length of the sample line.
The sample line should not bow or bend when a tube is loaded. If you need
to adjust the length, unscrew the nut on top of the sample injection
chamber, adjust the length, and tighten the nut again.
Changing the Air Filters
The BD FACSAria cytometer has two air filters: one in the sort collection
chamber door and one in the side door.
• To change the filter in the sort collection chamber door, slide out the old
filter and slide in the new filter. Refer to the documentation provided with
the Aerosol Management Option for more information.
• To change the air filter in the side door, turn the pegs at each corner of the
filter and remove the old filter. Install a new filter and turn the pegs to hold
the filter in place. Replace the filter once or twice a year, depending on the
quality of the air in the laboratory.
Changing the Bubble Filter
Change the bubble filter every 6 months or when increased debris appears in the
FSC vs SSC dot plot.
1 Reduce the sheath pressure to 10 psi.
2 Using a paper towel to cover the quick-disconnect coupling, disconnect the
sheath line from the filter outlet tubing at the top of the filter (Figure 6-6 on
page 204).
Make sure all compression fittings are securely tightened. If any fitting is
loose, the tubing could detach during high-pressure operation, exposing the
operator to potentially biohazardous sample spray.
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