Chapter 5: Sorting 171
Figure 5-7 Sort order on a slide
Setting Up the Stream
This section describes how to optimize side stream deflection and how to adjust
the Home location.
When sorting into a plate or onto a slide, the stage is pre-programmed to move a
set distance between wells on a plate or spots on a slide. The Home location is
used as the starting point. The far left stream should hit the center of the well in
the top left corner of a plate or the top left corner of a slide at the Home location.
Default Home location coordinates exist for each standard sort collection device:
Falcon multiwell plates with 6, 24, 48, 96, and 384 wells, and standard or frosted-
end slides. For other plate types, you will need to create a custom device. See
Creating a Custom Device on page 174.
Use the following procedure to verify the Home location and adjust it.
1 Optimize the drop delay.
See Determining the Drop Delay on page 158.
(A1)
(A2)
(A3)
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