Chapter 4: Running Samples 135
Setting Up the Workspace
Before you begin optimizing settings, it is important to verify the cytometer
configuration and create an experiment containing appropriate parameters for
the assay.
1 Select Cytometer > View Configurations, and verify the current
configuration is appropriate for the type of sample you are running.
2 Make sure the octagon and trigon(s) contain appropriate filters.
For assistance, see Application Options on page 250.
3 Click the corresponding buttons on the Workspace toolbar to display the
Browser ( ), Cytometer ( ), Inspector ( ), Worksheet ( ), and
Acquisition Dashboard ( ) windows, as needed.
4 (Optional) Create a folder for your experiment.
Select the icon for your database and press Ctrl-N; rename the folder
appropriately. Refer to the BD FACSDiva Software Reference Manual for
ideas on how to organize experiments.
Tip To place an experiment inside a folder, select the folder before you
create the experiment.
5 Create an experiment from the Doublet Discrimination Gating template.
For best results, BD Biosciences recommends that you perform doublet
discrimination in order to record only singlet events. This template contains
cytometer settings and gated plots for this purpose. A procedure is given in
Recording Data on page 145.
For accurate data results, the cytometer optics must match the current
cytometer configuration.
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