Chapter 6: Shutdown and Maintenance 205
Unscheduled Maintenance
There are several cytometer components that should be cleaned periodically or
checked for wear and replaced if necessary. See the indicated sections for the
following maintenance procedures.
Changing the Nozzle
As a general guideline, the nozzle opening should be at least three times the
diameter of the particle to be sorted. Two sizes of nozzles are provided with your
cytometer: 70- and 100-μm nozzles. The size is marked on the nozzle.
1 Turn off the stream and open the flow cell access door.
2 Turn the nozzle-locking lever to the left, and pull the nozzle out of the
cuvette flow cell (Figure 6-7 on page 206).
Procedure Recommended Frequency
Changing the Nozzle on this page As needed when running different size
particles
Adjusting the Nozzle Orifice on page 207 When satellite drops are not merging
properly
Cleaning the Nozzle on page 209 When stream irregularities indicate that
the nozzle is clogged
Cleaning the Camera Windows on
page 211
When smudges appear in the Breakoff or
Side Stream windows
Cleaning the Optical Filters on page 213 As needed when changing a filter
Purging the Bubble Filter on page 214 As needed to keep the flow cell clear of
bubbles
Any cytometer surface that comes in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
sorting hardware. Wear suitable protective clothing and gloves.
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