Chapter 5: Sorting 155
See Data Collection on page 134.
6 Install the required collection device and set up the side streams.
See Setting Up for Bulk Sorting on page 155 or Setting Up for Sorting Into a
Plate or Slide on page 169.
7 Calculate the drop delay.
See Determining the Drop Delay on page 158.
8 Use gating tools and subsetting methods to define the population(s) of
interest.
Examples of gating analysis can be found in Analyzing Data on page 146
and in Getting Started with BD FACSDiva Software.
9 Define a sort layout for the tube containing the defined sort populations
and proceed with sorting.
See Sorting on page 164.
Setting Up for Bulk Sorting
This section describes how to set up the streams for two- or four-way sorting. For
sorting using the ACDU option, see Setting Up for Sorting Into a Plate or Slide on
page 169.
1 Install collection tubes in the required collection device.
Collection tube holders are available for 1-mL microtubes, 12 x 75-mm tubes,
and 15-mL centrifuge tubes. For compatible tubes, see Labware on page 248.
2 Install the tube holder onto the cytometer.
Any cytometer surface that comes in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
sorting hardware. Wear suitable protective clothing and gloves.
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