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About This Guide
This user’s guide contains the instructions necessary to operate and maintain
your BD FACSAria™ flow cytometer. Because many instrument functions are
controlled by BD FACSDiva™ software, this guide also contains basic software
information needed for instrument setup. To familiarize yourself with the
software, do the tutorials in the Getting Started with BD FACSDiva Software
guide. For detailed information on software features, refer to the BD FACSDiva
Software Reference Manual.
The BD FACSAria User’s Guide assumes you have a working knowledge of basic
Microsoft® Windows® operation. If you are not familiar with the Windows
operating system, refer to the documentation provided with your computer.
New users of the BD FACSAria flow cytometer should read:
• Chapter 1 to become familiar with instrument components
• Chapter 2 to understand how the instrument works and to learn about the
software components used to control different subsystems
• Chapter 3 to see where software components are located
Instructions for routine acquisition, analysis, and sorting can be found in
Chapters 4 and 5.
Once you become familiar with routine operation and need only a quick
reminder of the main steps, use the quick reference guide provided with this
user’s guide.