36 BD FACSAria User’s Guide
Optics System
The BD FACSAria cytometer uses innovative designs for both the excitation
optics and collection optics. The optics can be viewed by opening the optics
access door and the flow cell access door. See the following sections for more
information.
Excitation Optics
The excitation optics consist of lasers, fiber optic cables, beam-shaping prisms,
and an achromatic focusing lens, as shown in Figure 1-15 on page 37. For
information about how signals are generated, see Signal Generation on page 50.
The BD FACSAria cytometer uses low-powered, air-cooled and solid-state lasers
that do not have special power and cooling requirements. The cytometer comes
standard with 488-nm and 633-nm lasers. A third 405-nm laser is available as an
option.
The flow cell access door is equipped with a shutter mechanism that shuts
off the laser light when the door is opened. To ensure there is no
interruption to data acquisition, do not open the door while sorting or
recording.
Laser
Wavelength
(nm)
Min. Power
a
(mW)
a. Measured out of fiber optic cable
Commonly Used Fluorochromes
or Dyes
Coherent® Sapphire™
Solid State
488 (blue) 13 FITC, PE
b
, PE-Texas Red®,
PerCP, PerCP-Cy
™5.5,
PE-Cy7, PI
b. Patents: PerCP—US 4,876,190; APC-Cy7—US 5,714,386
JDS Uniphase™ HeNe
Air Cooled
633 (red) 11 APC, APC-Cy7
Point Source Violet
Solid State (optional)
405 (violet) 30 Alexa Fluor®, Cascade Blue®,
Pacific Blue™, DAPI, Hoechst
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