BD FACSAria - Page 37

Chapter 1: BD FACSAria Cytometer Components 37
Fiber optics direct the laser light in a precise and constant manner onto beam-
shaping prisms, which in turn transmit the laser light to a focusing lens. The lens
focuses the laser light onto the sample core stream within the cuvette flow cell
(Figure 1-15). The lasers are positioned on the sample stream for optimal
generation of signals. Since the optical pathway and sample core stream are
fixed, optimization is constant from day to day.
Figure 1-15 Excitation optics pathway (behind laser shielding)
Lasers emit intense, coherent electromagnetic radiation that can cause
irreparable damage to human skin and eyes. To prevent retinal burns and
possible blindness, do not remove laser shielding, adjust laser controls, or
attempt to service the cytometer any place where laser warning labels are
attached. See Precaution Labels on page xviii for the placement of laser
warning labels.
fiber optics
prisms
prisms
cuvette
flow cell
focusing lens
upper
camera