84 BD FACSAria User’s Guide
Figure 3-2 Setting the sample temperature
NOTE The cooling/heating unit is designed to maintain the temperature of a
sample tube. It is not designed to cool or heat the sample. It takes approximately
45 minutes to reach the required temperature inside the chamber, during which
time the chamber must be kept closed.
To cool or heat the chamber, install a sample tube and click Load. Click Stop
Acquiring to keep the chamber closed and stop running the sample. To maintain
the temperature, do not leave the chamber open for extended periods while
changing sample tubes.
Fluidics Level Indicators
BD FACSDiva software provides fluidics level indicators in the Cytometer
window (Figure 3-3 on page 85). The sheath and waste indicators provide an
approximate indication of the fluid levels in each tank. The DI, bleach, and
ethanol tank indicators appear full until the fluid level descends below 20% of
the tank capacity. When this occurs, the corresponding level indicator changes to
black.
cooling/heating
unit for sample
injection chamber