Chapter 4: Running Samples 141
Data Recording and Analysis
Once you have optimized the cytometer electronics for your sample type, you are
ready to record and analyze data.
Before you record data, BD Biosciences recommends that you gate out doublets
in order to record only singlet events. The Doublet Discrimination Gating
template provides gated plots for this purpose.
During analysis, recorded data is displayed in plots, and gates are used to define
populations of interest. BD FACSDiva software analyzes the gated data and
calculates statistics that you can print or export. With global worksheets, data
can be shown for a series of tubes on the same worksheet, manually or in an
automated batch analysis.
The following sections describe how to use BD FACSDiva software features to
record and analyze sample data.
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