220 BD FACSAria User’s Guide
Troubleshooting the Stream
Observation Possible Causes Recommended Solutions
Stream not in center
of aspirator
Difference in keyed stream
position between nozzles
If you have just changed the
nozzle, use an Allen wrench to
loosen the screws on either side of
the sort block. Adjust the angle of
the sort block until the stream
flows into the center of the waste
aspirator, and then tighten the
screws. See Figure 1-11 on
page 32.
Nozzle inserted improperly Turn off the stream. Remove the
nozzle and ensure that the O-ring
is in place. Re-insert the nozzle at
a slight downward angle to
prevent loss or movement of the
O-ring. Make sure the nozzle is
completely seated against the back
wall of the cuvette flow cell.
Clogged or damaged nozzle Turn off the stream, remove the
nozzle, and examine the nozzle tip
under a microscope.
• If debris is visible, clean the
nozzle. See Cleaning the Nozzle
on page 209.
• If the nozzle appears damaged,
replace it. See Changing the
Nozzle on page 205.
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