Chapter 7: Troubleshooting 221
No stream or dripping
stream
Nozzle inserted improperly Turn off the stream. Remove the
nozzle. See Changing the Nozzle
on page 205 for instructions.
Clogged or damaged nozzle Turn off the stream, remove the
nozzle, and examine the nozzle tip
under a microscope.
• If debris is visible, clean the
nozzle. See Cleaning the Nozzle
on page 209.
• If the nozzle appears damaged,
replace it. See Changing the
Nozzle on page 205.
Stream control disabled
or no stream when
Stream control clicked
Plenum is not full Wait for the plenum to fill.
Air lock in bubble or fluidics
filter
Prime the system with the
corresponding fluid.
If the control is still disabled,
remove the filter, install bypass
tubing, and repeat the priming
procedure until you see fluid in
the line.
When fluid is running through the
line, remove the bypass tubing,
install the filter, and repeat the
priming procedure one last time.
Communication failure
between workstation and
cytometer
Quit the software and restart it.
Troubleshooting the Stream (continued)
Observation Possible Causes Recommended Solutions
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