222 BD FACSAria User’s Guide
No stream when Stream
control clicked
Air in the bubble filter Purge the bubble filter. See
Purging the Bubble Filter on
page 214.
Sheath container low or
empty
Refill the sheath tank. See
Refilling Containers on page 113.
Note that when the empty tank
warning message is not dismissed
after 15 minutes, the stream shuts
off automatically.
Air in sheath line Prime the sheath tank. See Prime
After Tank Refill on page 185.
Air in sheath filter Purge the sheath filter. See Purging
Filters on page 198.
Dry filter 1 Install bypass tubing in place of
the filter for the affected fluid,
then run Prime After Tank
Refill.
a Re-install the filter and open
the bleeder valve to purge the
filter. See Purging Filters on
page 198.
Fanning around center
stream
Nozzle inserted improperly Re-insert the nozzle. Push it gently
all the way forward without
rocking it from side to side.
Unstable stream Debris in flow cell or nozzle Remove the nozzle and run the
stream with no nozzle in place for
approximately 10 seconds. (Click
the Stream control on, then off.)
Sonicate the nozzle and re-install
it.
Troubleshooting the Stream (continued)
Observation Possible Causes Recommended Solutions
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