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BD FACSAria - Page 223

BD FACSAria
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Chapter 7: Troubleshooting 223
Leaking or spraying
around nozzle
Defective nozzle O-ring Replace the O-ring.
Nozzle inserted improperly Turn off the stream. Remove the
nozzle. See Changing the Nozzle
on page 205 for instructions.
Extra O-ring is blocking the
nozzle
Remove the nozzle and use a
cotton swab to clear out the
cuvette.
Drop breakoff is too
long
Bubbles in flow cell Open the flow cell access door
and check for bubbles in the flow
cell. If they are visible, turn off the
stream, wait a few seconds, and
turn on the stream again.
Attenuation is on Turn off attenuation.
Amplitude is too low Increase the amplitude until you
can see drops. If you need a very
high amplitude (>70 volts) to see
drops, you might have air bubbles
in the flow cell.
Nozzle inserted improperly Turn off the stream. Remove the
nozzle and ensure that the O-ring
is in place. Re-insert the nozzle at
a slight downward angle to
prevent loss or movement of the
O-ring. Make sure the nozzle is
completely seated against the back
wall of the cuvette flow cell.
Troubleshooting the Stream (continued)
Observation Possible Causes Recommended Solutions

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