166 BD FACSAria User’s Guide
• Change the sort precision mode to Purity (two tubes), 4-Way Purity
(four tubes), or Single Cell (plate or slide).
• Enter the number of target events by choosing a value from the drop-
down menu or entering a number in the field.
• Select Save Conflicts if you are using a 2- or 4-Tube Sort Layout and
want to save conflicts.
• Select the sort location field(s) to be sorted into. Select multiple fields
by dragging the mouse. Select a row or column by clicking the row or
column header.
• Add the required population(s) to each sort location field.
• To display fewer counters in the Sort Layout window, click the View
Counters button and deselect a menu option. The corresponding
counter is hidden. (Only counters with a checkmark next to the name
are displayed.)
Starting and Monitoring the Sort
1 Open the sort collection chamber door and install the collection tubes,
plate, or slide.
2 Install the sample tube onto the loading port and click Load.
3 Adjust the required flow rate, if necessary.
Typically, flow rates less than 6 give the best sorting results.
4 (Optional) Turn on the deflection plates and open the aspirator drawer.
The deflection plates turn off automatically each time a tube is unloaded. If
you do not turn them back on before beginning a sort, a dialog box appears
where you can turn on the plates and open the aspirator drawer by clicking
OK.
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