Chapter 4: Running Samples 143
Setting Up the Global Worksheet
A global worksheet will be used to perform doublet discrimination, and to set up
plots to preview and record data.
1 Click the Worksheets View button ( ) on the Worksheet toolbar.
The global worksheet is shown. If you are using the Doublet
Discrimination Gating template, the worksheet shows the predefined plots
and gates used to distinguish singlets from doublets.
The second and third plots are set up to display gated data from the first
and second plots, respectively, as shown in the population hierarchy.
2 Create two dot plots for previewing and recording data.
For this example, create a FITC vs PE plot and an APC vs PerCP-Cy5.5
plot.
Tip Double-click the Plot button to keep it selected until you create all plots.
Click any other button to undo the selection.
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