153
5
Sorting
You can program BD FACSDiva software to sort a specified number of particles
from multiple populations into a variety of sorting devices, including tubes,
plates, and slides. Collection devices are provided for sorting into 1-mL,
12 x 75-mm, and 15-mL tubes; hardware for sorting into plates and slides is
available as an option. Up to four defined populations can be sorted into each
tube, allowing up to 16 populations to be sorted at one time.
Any subsetted population can be used for sorting, including populations defined
by quadrant gates, interval gates, or derived gates. A single sort population can
be defined by up to eight gates.
The following topics are covered in this chapter:
• Setting Up for Sorting on page 154
• Determining the Drop Delay on page 158
• Sorting on page 164
• Setting Up for Sorting Into a Plate or Slide on page 169
Before you begin, you should be familiar with BD FACSAria operation using
BD FACSDiva software. Review chapters 2 and 3, if needed.
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