118 BD FACSAria User’s Guide
• Track instrument performance using Levey-Jennings plots.
• Detect trends and identify potential cytometer problems.
Refer to the Cytometer Setup and Tracking Application Guide for details on the
reasons for using CS&T, and which types of applications will benefit the most
from using it.
Cytometer Quality Control Using BD FACSDiva
Software
Perform cytometer quality control (QC) to ensure consistent performance over
time. During cytometer QC, you want to keep as many variables as constant as
possible. For example, always use the same QC particle type and lot number,
sheath pressure, and PMT settings. If you work with different sheath pressures,
you might consider having a separate QC experiment for each pressure.
When the experiment, cytometer settings, and QC sample are constant, changes
in the recorded means and coefficients of variation (CVs) indicate changes in
cytometer performance over time. QC data should be analyzed for trends over
30–60 runs.
This section describes how to use a QC experiment template to verify laser delay
and area scaling values, and how to record parameter means and CVs for a
fluorescent bead. For examples of fluorescent particles that can be used for
cytometer QC, see Cytometer Setup Particles on page 246.
NOTE QC results are affected by laser and fluidics performance. BD strongly
recommends following the laser and fluidics maintenance procedures in
Chapter 6.
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