58 BD FACSAria User’s Guide
Detectors
Detectors within each detector array convert light signals into electrical signals
that can be processed by the electronics system.
There are two types of signal detectors in the BD FACSAria flow cytometer: the
photodiode and photomultiplier tubes (PMTs). The photodiode is less sensitive to
light signals than the PMTs, thus is used to detect the stronger FSC signal. The
photodiode detects FSC light from the blue laser, and is stored outside the
detector arrays. PMTs are used to detect the weaker signals generated by SSC and
all fluorescence channels. The octagon can hold up to eight PMTs; each trigon
can contain up to three PMTs.
Each PMT detects only one fluorochrome at a time. In BD FACSDiva software,
the Cytometer Configuration window lets you define which fluorochromes or
cell parameters will be measured at each PMT detector. If more than one
fluorochrome is measured using the same PMT, you can add additional
parameters to your configuration and choose the appropriate fluorochrome
within your software experiment. See Cytometer Configuration on page 86 for
more information.
Figure 2-11 Example BD FACSAria cytometer configuration
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