94 BD FACSAria User’s Guide
• Home Device—Opens a dialog box containing commands to move the tray
support arm, either manually or to the home position. See Setting Up the
Stream on page 171.
• Custom Devices—Opens a dialog box where custom devices can be
defined. See Creating a Custom Device on page 174.
• Sort Report—Displays a report showing the sort settings, acquisition
counters, and sort layout information from the current sort. See Sort
Report on page 103.
Sort Setup
Sort setup values for four different nozzle sizes can be downloaded using the Sort
> Sort Setup command. Default settings are provided for each sorting option,
along with a custom setting for each option. Use the following guidelines to
determine which option to use. As a general rule, for optimal results when sorting
large or fragile cells, use a larger nozzle size and lower pressure. To increase
throughput and yield when sorting smaller or less fragile cells, use a smaller
nozzle size and higher pressure.
If you make changes to any of the settings, the changes are automatically saved
when you exit BD FACSDiva software or when you switch to a different sort
setup mode. When you restart, the most recently used set of values is restored.
Default settings are shown in Table 3-1 on page 95.
In addition, the sort setup menu has following menu items: 70 Custom, 85
Custom, 100 Custom, and 130 Custom. Initially, all of these new setups are exact
Sort Setup name Nozzle (micron) Default pressure (psi)
70 micron 70 70
85 micron
a
a. Contact your BD Sales representative for information on the availability of the
85 and 130 micron nozzles.
85 45
100 micron 100 20
130 micron
a
130 10
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