178 BD FACSAria User’s Guide
Daily Shutdown
Run the following procedures at the end of each day or when required:
• Fluidics Shutdown on this page
• External Cleaning on page 180
Fluidics Shutdown
At the end of each day the cytometer is used, run fluidics shutdown to remove
sheath fluid from the sheath path and rinse all lines with DI water. This prevents
saline crystals from clogging the fluidics.
1 Unload the sample tube, if one is loaded.
2 Open the flow cell access door.
3 Turn off the stream.
4 Select Cytometer > Fluidics Shutdown, and then OK.
5 Empty the waste and refill the DI water, if prompted to do so.
See Setting Up the Fluidics Cart on page 112. If you disconnect the DI
water tank for refilling, prime the system before continuing.
The cytometer starts the fluidics shutdown cycle. This removes sheath from
the lines and plenum and replaces it with DI water.
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