Safety and Limitations xvii
For information on laboratory safety, refer to the following guidelines.
• Schmid I, Nicholson JKA, Giorgi JV, et al. Biosafety guidelines for sorting
of unfixed cells. Cytometry. 1997; 28:99-117.
• Protection of Laboratory Workers From Occupationally Acquired
Infections; Approved Guideline – Third Edition. Wayne, PA: Clinical and
Laboratory Standards Institute, 2005. CLSI document M29-A3.
• Procedures for the Handling and Processing of Blood Specimens; Approved
Guideline – Third Edition. Wayne, PA: Clinical and Laboratory Standards
Institute; 2004. CLSI document H18-A3.
The two CLSI documents listed above can be ordered online at
www.clsi.org.
• European Council Directive on the protection of workers from risks related
to exposure to biological agents at work (90/679/EEC) and its relevant
national transpositions.
General Safety
• Keep your hands and clothing away from the sample injection chamber
when a tube is loading or unloading. Do not place objects underneath the
sample injection chamber.
Cell sorters that use droplet generation methods, like the BD FACSAria,
can produce aerosols around the sample stream. When acquiring
biohazardous samples, follow universal precautions at all times. Keep the
sort block door closed during sorting. If you need to access the sort block,
turn off the stream before opening the door.
Movement of mechanical parts within the instrument can pinch or injure
your hands or fingers. To prevent injury by moving parts, follow these
precautions.
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