158 BD FACSAria User’s Guide
7 Click the Voltage control ( ) to turn off the deflection plates.
Determining the Drop Delay
BD FACS Accudrop technology is used to determine the optimal drop delay
setting for your sorting application. For more information, see Drop Delay
Overview on page 69.
There are two methods for determining the drop delay.
• Manual drop delay using the standard method, as described in the
following sections.
• Auto drop delay using an automated algorithm method. See Determining
the Drop Delay - Automatic Method on page 162.
NOTE Before beginning this procedure, make sure the stream is stable and the
Sweet Spot is on.
Setting Up the Experiment
The steps in this section show you how to set the drop delay using the Accudrop
experiment template. Because no data is recorded, the experiment can be reused
as often as you like.
1 Create an experiment from the Accudrop Drop Delay template.
Select Experiment > New Experiment. Select the Accudrop Drop Delay
experiment and click OK.
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