214 BD FACSAria User’s Guide
Purging the Bubble Filter
The bubble filter is a standard 0.2-µm fluid filter that helps keep air bubbles and
small particles from reaching the flow cell. To maintain the filter, periodically
purge the air that accumulates in it.
During normal cytometer operation, the outlet tubing at the top of the bubble
filter is connected to the sheath line leading to the flow cell. To purge the filter,
disconnect the sheath line from the filter outlet tubing and connect the sheath line
to the filter purge tubing.
1 Reduce the sheath pressure.
• Select Cytometer > Sheath Pressure.
• Take note of the current sheath pressure—you will need to return the
value to this setting after the filter purge is complete.
• Change the pressure to 10 psi and click OK.
2 Using a paper towel to cover the coupling, disconnect the sheath line from
the filter outlet tubing by pressing the tab on the quick-disconnect coupling
(Figure 6-10 on page 215).
Moisture in electrical circuitry can result in a short-circuit or present a
shock hazard. To prevent fluid from spilling onto electrical components,
wrap connectors loosely in paper towels before disconnecting any fluid
lines. Use paper towels to absorb the excess fluid.
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