Chapter 7: Troubleshooting 225
Sorting Troubleshooting
Observation Possible Causes Recommended Solutions
Unstable breakoff while
Sweet Spot is engaged
Residual ethanol in system Allow the system to run until the
breakoff stabilizes.
Target Drop 1 value is out of
range for drop spacing
Use an actual Drop 1 value for the
target. Remember to repeat the
drop delay setup each time the
target value is changed.
Nozzle is clogged or
inserted improperly
Turn off the stream. Remove the
nozzle and clean it. re-insert the
nozzle at a slight downward angle
to prevent loss or movement of the
O-ring. Make sure the nozzle is
completely seated against the back
wall of the cuvette flow cell.
Dirty strobe lens or upper
camera window
Clean the lens and the window as
described in Cleaning the Camera
Windows on page 211.
Air lock in bubble or fluidics
filter
Prime the system with the
corresponding fluid.
If the control is still disabled,
remove the filter, install bypass
tubing, and repeat the priming
procedure until you see fluid in
the line.
When fluid is running through the
line, remove the bypass tubing,
install the filter, and repeat the
priming procedure one last time.
Debris in flow cell or nozzle Remove the nozzle and run the
stream with no nozzle in place for
approximately 10 seconds. (Click
the Stream control on, and then
off.) Sonicate the nozzle and
re-install it.
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